### Pipeline run code and environment:
* Command: `/home/mjs5kd/.local/bin/refgenie build hg38_chr22/bowtie2_index`
* Compute host: udc-ba25-18
* Working dir: /sfs/qumulo/qproject/shefflab/deploy/rg.databio.org_full/rg.databio.org
* Outfolder: /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/_refgenie_build/
* Pipeline started at: (04-07 16:26:40) elapsed: 0.0 _TIME_
### Version log:
* Python version: 3.6.6
* Pypiper dir: `/sfs/qumulo/qhome/mjs5kd/.local/lib/python3.6/site-packages/pypiper`
* Pypiper version: 0.12.1
* Pipeline dir: `/sfs/qumulo/qhome/mjs5kd/.local/bin`
* Pipeline version: None
### Arguments passed to pipeline:
* `asset_registry_paths`: `['hg38_chr22/bowtie2_index']`
* `assets`: `None`
* `command`: `build`
* `config_file`: `refgenie.yaml`
* `docker`: `False`
* `files`: `None`
* `genome`: `None`
* `genome_config`: `None`
* `genome_description`: `None`
* `logdev`: `False`
* `new_start`: `False`
* `outfolder`: `/project/shefflab/deploy/rg.databio.org_full/genomes/data`
* `params`: `None`
* `recipe`: `None`
* `recover`: `False`
* `requirements`: `False`
* `silent`: `False`
* `skip_read_lock`: `False`
* `tag_description`: `None`
* `verbosity`: `None`
* `volumes`: `None`
----------------------------------------
Target to produce: `/project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/_refgenie_build/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7_bowtie2_index__default.flag`
> `bowtie2-build /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/fasta/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.fa /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7` (32085)
[33mWARNING:[0m Skipping mount /opt/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container
Settings:
Output files: "/project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.*.bt2"
Line rate: 6 (line is 64 bytes)
Lines per side: 1 (side is 64 bytes)
Offset rate: 4 (one in 16)
FTable chars: 10
Strings: unpacked
Max bucket size: default
Max bucket size, sqrt multiplier: default
Max bucket size, len divisor: 4
Difference-cover sample period: 1024
Endianness: little
Actual local endianness: little
Sanity checking: disabled
Assertions: disabled
Random seed: 0
Sizeofs: void*:8, int:4, long:8, size_t:8
Input files DNA, FASTA:
/project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/fasta/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.fa
Building a SMALL index
Reading reference sizes
Time reading reference sizes: 00:00:01
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:00
bmax according to bmaxDivN setting: 10673160
Using parameters --bmax 8004870 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 8004870 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:01
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:01
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:00
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 4.26926e+07 (target: 8004869)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 1
No samples; assembling all-inclusive block
Sorting block of length 42692640 for bucket 1
(Using difference cover)
Sorting block time: 00:00:26
Returning block of 42692641 for bucket 1
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 11345849
fchr[G]: 21309057
fchr[T]: 31392427
fchr[$]: 42692640
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 18433203 bytes to primary EBWT file: /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.1.bt2
Wrote 10673168 bytes to secondary EBWT file: /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 42692640
bwtLen: 42692641
sz: 10673160
bwtSz: 10673161
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 2668291
offsSz: 10673164
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 222358
numLines: 222358
ebwtTotLen: 14230912
ebwtTotSz: 14230912
color: 0
reverse: 0
Total time for call to driver() for forward index: 00:00:35
Reading reference sizes
Time reading reference sizes: 00:00:01
Calculating joined length
Writing header
Reserving space for joined string
Joining reference sequences
Time to join reference sequences: 00:00:00
Time to reverse reference sequence: 00:00:00
bmax according to bmaxDivN setting: 10673160
Using parameters --bmax 8004870 --dcv 1024
Doing ahead-of-time memory usage test
Passed! Constructing with these parameters: --bmax 8004870 --dcv 1024
Constructing suffix-array element generator
Building DifferenceCoverSample
Building sPrime
Building sPrimeOrder
V-Sorting samples
V-Sorting samples time: 00:00:01
Allocating rank array
Ranking v-sort output
Ranking v-sort output time: 00:00:00
Invoking Larsson-Sadakane on ranks
Invoking Larsson-Sadakane on ranks time: 00:00:01
Sanity-checking and returning
Building samples
Reserving space for 12 sample suffixes
Generating random suffixes
QSorting 12 sample offsets, eliminating duplicates
QSorting sample offsets, eliminating duplicates time: 00:00:00
Multikey QSorting 12 samples
(Using difference cover)
Multikey QSorting samples time: 00:00:00
Calculating bucket sizes
Splitting and merging
Splitting and merging time: 00:00:00
Avg bucket size: 4.26926e+07 (target: 8004869)
Converting suffix-array elements to index image
Allocating ftab, absorbFtab
Entering Ebwt loop
Getting block 1 of 1
No samples; assembling all-inclusive block
Sorting block of length 42692640 for bucket 1
(Using difference cover)
Sorting block time: 00:00:24
Returning block of 42692641 for bucket 1
Exited Ebwt loop
fchr[A]: 0
fchr[C]: 11345849
fchr[G]: 21309057
fchr[T]: 31392427
fchr[$]: 42692640
Exiting Ebwt::buildToDisk()
Returning from initFromVector
Wrote 18433203 bytes to primary EBWT file: /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.rev.1.bt2
Wrote 10673168 bytes to secondary EBWT file: /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7.rev.2.bt2
Re-opening _in1 and _in2 as input streams
Returning from Ebwt constructor
Headers:
len: 42692640
bwtLen: 42692641
sz: 10673160
bwtSz: 10673161
lineRate: 6
offRate: 4
offMask: 0xfffffff0
ftabChars: 10
eftabLen: 20
eftabSz: 80
ftabLen: 1048577
ftabSz: 4194308
offsLen: 2668291
offsSz: 10673164
lineSz: 64
sideSz: 64
sideBwtSz: 48
sideBwtLen: 192
numSides: 222358
numLines: 222358
ebwtTotLen: 14230912
ebwtTotSz: 14230912
color: 0
reverse: 1
Total time for backward call to driver() for mirror index: 00:00:32
Command completed. Elapsed time: 0:01:08. Running peak memory: 0.265GB.
PID: 32085; Command: bowtie2-build; Return code: 0; Memory used: 0.265GB
> `touch /project/shefflab/deploy/rg.databio.org_full/genomes/data/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index/default/_refgenie_build/fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7_bowtie2_index__default.flag` (32518)
Command completed. Elapsed time: 0:00:00. Running peak memory: 0.265GB.
PID: 32518; Command: touch; Return code: 0; Memory used: 0.001GB
Asset digest: f756f8dd195756b78e2f9dfbb9146fcf
Default tag for 'fec6bcce9821b57f98ffc5d46ebec4f2f756c2e9db800ab7/bowtie2_index' set to: default
### Pipeline completed. Epilogue
* Elapsed time (this run): 0:01:11
* Total elapsed time (all runs): 0:01:09
* Peak memory (this run): 0.2654 GB
* Pipeline completed time: 2021-04-07 16:27:51
Finished building 'bowtie2_index' asset
Created alias directories:
- /project/shefflab/deploy/rg.databio.org_full/genomes/alias/hg38_chr22/bowtie2_index/default