[2021-05-13 18:17:54.038] [puff::index::jointLog] [info] Running fixFasta [2021-05-13 18:17:55.352] [puff::index::jointLog] [warning] Entry with header [ENSRNOT00000085980.1], had length less than equal to the k-mer length of 31 (perhaps after poly-A clipping) [2021-05-13 18:17:55.512] [puff::index::jointLog] [warning] Entry with header [ENSRNOT00000093397.1], had length less than equal to the k-mer length of 31 (perhaps after poly-A clipping) [2021-05-13 18:18:43.886] [puff::index::jointLog] [warning] Removed 521 transcripts that were sequence duplicates of indexed transcripts. [2021-05-13 18:18:43.886] [puff::index::jointLog] [warning] If you wish to retain duplicate transcripts, please use the `--keepDuplicates` flag [2021-05-13 18:18:43.886] [puff::index::jointLog] [warning] There were 2 duplicate decoy sequences. [2021-05-13 18:18:43.895] [puff::index::jointLog] [info] Replaced 140324814 non-ATCG nucleotides [2021-05-13 18:18:43.895] [puff::index::jointLog] [info] Clipped poly-A tails from 58 transcripts [2021-05-13 18:19:09.168] [puff::index::jointLog] [info] Filter size not provided; estimating from number of distinct k-mers [2021-05-13 18:19:35.557] [puff::index::jointLog] [info] ntHll estimated 2411746066 distinct k-mers, setting filter size to 2^36 [2021-05-13 19:38:55.016] [puff::index::jointLog] [info] Starting the Pufferfish indexing by reading the GFA binary file. [2021-05-13 19:38:55.017] [puff::index::jointLog] [info] Setting the index/BinaryGfa directory /project/shefflab/deploy/rg.databio.org_full/genomes/data/79168fd950d561cf5ce454dd27593c0af429b32cddce5ce9/salmon_sa_index/default [2021-05-13 19:39:00.779] [puff::index::jointLog] [info] Done wrapping the rank vector with a rank9sel structure. [2021-05-13 19:39:01.152] [puff::index::jointLog] [info] contig count for validation: 24844458 [2021-05-13 19:39:17.250] [puff::index::jointLog] [info] Total # of Contigs : 24844458 [2021-05-13 19:39:17.250] [puff::index::jointLog] [info] Total # of numerical Contigs : 24844458 [2021-05-13 19:39:18.620] [puff::index::jointLog] [info] Total # of contig vec entries: 268543925 [2021-05-13 19:39:18.620] [puff::index::jointLog] [info] bits per offset entry 29 [2021-05-13 19:39:33.846] [puff::index::jointLog] [info] Done constructing the contig vector. 24844459 [2021-05-13 19:40:09.480] [puff::index::jointLog] [info] # segments = 24844458 [2021-05-13 19:40:09.480] [puff::index::jointLog] [info] total length = 3171654430 [2021-05-13 19:40:09.661] [puff::index::jointLog] [info] Reading the reference files ... [2021-05-13 19:40:26.934] [puff::index::jointLog] [info] positional integer width = 32 [2021-05-13 19:40:26.934] [puff::index::jointLog] [info] seqSize = 3171654430 [2021-05-13 19:40:26.934] [puff::index::jointLog] [info] rankSize = 3171654430 [2021-05-13 19:40:26.934] [puff::index::jointLog] [info] edgeVecSize = 0 [2021-05-13 19:40:26.934] [puff::index::jointLog] [info] num keys = 2426320690 [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] mphf size = 1515.54 MB [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk size = 396456804 [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 0 = [0, 396456804) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 1 = [396456804, 792913608) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 2 = [792913608, 1189370412) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 3 = [1189370412, 1585827242) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 4 = [1585827242, 1982284046) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 5 = [1982284046, 2378740850) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 6 = [2378740850, 2775197654) [2021-05-13 19:48:22.938] [puff::index::jointLog] [info] chunk 7 = [2775197654, 3171654400) [2021-05-13 19:59:22.913] [puff::index::jointLog] [info] finished populating pos vector [2021-05-13 19:59:22.913] [puff::index::jointLog] [info] writing index components [2021-05-13 20:00:19.389] [puff::index::jointLog] [info] finished writing dense pufferfish index